PCR cleanup

PCRNiborlab PCR Clean Up Miniprep System contains a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5-5.5) and elution of DNA with low-salt buffer (pH 7.0-9.0) or ddH2O. The purification procedure removes primers, nucleotides, enzyme, salts, ethidium bromide and other impurities from DNA samples.

Features

  • Clean up DNA in 5 minutes
  • Up to 95% recovery of DNA fragment (100 bp to 10 kb)
  • High purity of DNA (A260 /A280 >1.9)
  • > 95% primer and salts removal
  • Elute DNA with just 15-30 ul Elution buffer or ddH2O
  • Available sizes: 50 preps, 250 preps

Downstream applications

Niborlab PCR Clean Up Miniprep System provides reproducible yields of high-purity DNA suitable for use in most applications, including:

  • Automated fluorescent and radioactive sequencing & PCR
  • Restriction digestion & modifying enzymatic reaction
  • Ligation
  • Labeling and hybridization