In cell biology, transfection is the term given to the introduction of foreign genetic material into eukaryotic cells. In addition, the distinction is made between temporary introduction into the host cell (transient transfection) and permanent integration into the genome (stable transfection). The process of transfection basically corresponds to that of bacterial transformation, albeit under a different name.
- 1 mL: $396 / 289 €
- 2 mL: $729 / 536 €
- 5 mL: $1700 / 1250 €
Direct links to transfection protocols:
- MEF transfection
- HEK293 transfection
- HUVEC transfection
- Hep G2 transfection
- Other cell types: Lipotransfectin manual
A variety of methods are used to introduce genetic material into eukaryotic cells: viral transfection uses genetically modified viruses which are no longer pathogenic and carry the gene to be introduced. In this method problems include the frequently non-directional stable integration of the gene into the genome of the host cell, the labor-intensive production of the viruses and the strong immunological response.
Physical methods such as electroporation or microinjection are cost-intensive and of limited use in routine procedures. Chemical methods include calcium phosphate precipitation, transfection using cationic polymers and – among the most efficient methods – lipotransfection.
Transfection with cationic lipids
In aqueous solutions, cationic lipids form vesicles with a bilayer lipid sheet, known as liposomes. When liposomes encounter nucleic acids they re-form into nucleic acid-lipid complexes called lipoplexes which can be actively taken up by eukaryotic cells by means of endocytosis. In this case, the lipoplex enters into the cell cytosol via the endosomes.
The endosomal structure is destroyed by increasing the osmotic pressure created by the lipids’ buffering action within the endosomes and by the fusion of the lipid with the endosomal membrane. The ability of a lipid to destroy endosomes is one of the main characteristics of a transfection reagent. Our Lipotransfectin transfection reagent features structural elements designed to optimally promote precisely these properties.
DNA which is introduced into the cytosol cannot penetrate the nuclear membrane. Access to the nucleus is, thus, only possible if the nuclear membrane dissolves during mitosis. For this reason, the cell division rate is critical in DNA transfection and must be as high as possible for efficient transfection.
Lipotransfectin tranfection reagent
Lipotransfectin is a highly advanced transfection reagent which sets new standards in eukaryotic cell transfection. In addition, its low cytotoxicity and freedom from serum inhibition make it suitable for a wide range of uses.
Lipotransfectin is suitable for an almost unlimited range of uses, from highly efficient plasmid and bacmid transfections to siRNA transfection or high-throughput screening systems.
Even unusual applications such as transfection of frog (Xenopus) and insect (Plutella xylostella, Spodoptera exigua, etc.) cells or bacmid transfections can be completed successfully.
|Application||Transfection of mammalian cells with nucleic acids|
|Formulation||Cationic lipids with colipids in water|
|Assays||Up to 1500 (24-well) or up to 400 (6-well) per 1 ml reagent|
|Shipping||At room temperature|
|Shelf Life||1 year (after date of delivery)|